Monoecious and andromonecious nature in bottle gourd leads to higher levels of cross pollination. This mechanism favours wide diversity. Several varieties were evolved through selection from the native germplasm in bottle gourd. However, development of hybrids with high yield by using heterosis breeding is need of the hour. Conventional crop improvement strategies take longer time and require huge man power for the development of inbreds used as parents. Tissue culture techniques are being followed in vegetables to minimize the time taken for the development of inbred. Doubled haploids are developed through regeneration of haploid plants by using tissue culture protocols. Subsequently, these haploid plants are developed into homozygous conditions by doubling of chromosomes. It is time saving process and minimizes the time and resources required for raising many generations. Several studies have been conducted in cucumber, squash and muskmelon. However, few regeneration protocols are available in bottle by using cotyledons as explant. So, there is an immediate need for standardization of haploid development protocols in bottle gourd as similar to other Cucumis species.
Development, Inbred Lines, Hybrids Development, Bottle Gourd, Lagenaria siceraria, Doubled Haploid Technique
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